Comparison of the Biological Behaviour of Human Dermal Fibroblasts seeded on 3D Printed Polylactic acid, Polycaprolactone and Polyethylene Terephthalate Scaffolds in vitro

Regenerative medicine is a scientific field that improves and repairs diseased injured tissues. Three-dimensional (3D) printing an innovative technology provides new application for regenerative medicine. 3D printed scaffolds by programming pore sizes shapes serve as temporary basis cells until the natural extracellular matrix (ECM) reconstructed. Dermal fibroblasts are mesenchymal located in dermal skin layer produce organize ECM components. They play essential role wound healing fibrosis. The aim of this study to analyze viability, adhesion, distribution, collagen IV expression human (HDFs) seeded on polylactic acid (PLA), polyethylene terephthalate (PET), poly-ε-caprolactone (PCL) vitro. HDFs were or tissue culture plastic plates control cultured 1 3 days. PLA, PCL, PET prepared using custom made fused deposition modeling printer. cell viability was measured WST-1 assay days 3. adhesion evaluated scanning electron microscopy (SEM). distribution analyzed hematoxylin eosin (H&E) staining. Collagen immunohistochemical (IHC) On day 1, PLA significantly higher than PCL scaffolds. 3, SEM images showed attached surfaces, filled interfiber gaps maintained their specific morphology compared Histological stained with H&E demonstrated uniform staining more intense This shows create appropriate environment may provide high advantage regeneration.